A simple spectrophotometric assay of carboxypeptidase N (kininase I) in human serum.

The spectrophotometric determination of human serum carboxypolypeptidase with angiotensin converting enzyme-like activity.

1. A colorimetric method was developed for the direct chemical assay of human carboxypeptidase A (carboxypolypeptidase; EC 3.4.12.2) with angiotensin converting enzyme-like activity in serum or plasma, with the substrate analogue glycyl-L-histidylglycine and the angiotensin converting enzyme substrate angiotensin I (A-I). This method was based on the spectrophototometric determination of histid...

Regulation of Cardiovascular Signaling by Kinins and Products of Similar Converting Enzyme Systems Kininase I-type carboxypeptidases enhance nitric oxide production in endothelial cells by generating bradykinin B1 receptor agonists

Sangsree, Sakonwun, Viktor Brovkovych, Richard D. Minshall, and Randal A. Skidgel. Kininase I-type carboxypeptidases enhance nitric oxide production in endothelial cells by generating bradykinin B1 receptor agonists. Am J Physiol Heart Circ Physiol 284: H1959–H1968, 2003. First published March 6, 2003; 10.1152/ajpheart.00036.2003. Kininase I-type carboxypeptidases convert native kinin agonists ...

A labile enzyme in fresh human serum interferes with the assay of carboxypeptidase N.

Preparation of an artificial matrix to replace human standards of prostate specific antigen IRMA assay kit [Persian]

  Introduction: Prostate specific antigen (PSA) is one of the most sensitive markers for diagnosis of prostate cancer. Immunoradiometric kit (IRMA) is a common and sensitive method for determination of PSA in clinical laboratories. This kit has four major components: solid phase coated with monocolonal antibody, pair antibody labelled with I-125, series of standards i...

Radioimmunoassay of active pancreatic enzymes in sera from patients with acute pancreatitis. I. Active carboxypeptidase B.

Previous studies have suggested that measurement of active enzymes in relation to proenzymes in serum of patients with pancreatitis may reflect the degree of zymogen activation in the gland. Here we describe the first single-tube assay for an active form of a pancreatic enzyme that is ordinarily synthesized as a proenzyme. Human procarboxypeptidase B, which we purified to near homogeneity, is a...